Journal: medRxiv

Article Title: Polymorphism in IFNAR contributes to glucocorticoid response and outcome in ARDS and COVID-19

doi: 10.1101/2022.03.10.22272123

Figure Lengend Snippet: (A ) STAT1 expression in the lung after 4-day culture in the presence of IFN beta with or without hydrocortisone (HC). ( B) pSTAT1 expression in the same specimens as in A. ( C) Example photomicrographs showing higher STAT2 expression in a TT patient than in a CT patient and the effect of HC on its nuclear translocation. Most STAT2 remains in the cytoplasm of the CT patients, whereas nuclear expression is prominent in the TT patient. Indicated insets are shown in the bottom row. Arrows. ( D ) Combined results of all patients noting that two CT samples are excluded in the data as the patients were already under glucocorticoid treatment at the time of sample acquisition. Ns, not significant; *P<0.05; **P<0.01; and ***P<0.001

Article Snippet: The first stage antibodies were anti-alpha chain of the IFN alpha/beta receptor (St John’s Laboratory STJ112765) that was used 1:2000 and 1:5000 and anti-Stat1 (1:400, 9175S), anti-pStat1(1:100, 9167S) and anti-Stat2 (1:200, 72604S) all from Cell Signalling.

Techniques: Expressing, Translocation Assay

Journal: Antioxidants

Article Title: Exo70 Protects Against Memory and Synaptic Impairments Following Mild Traumatic Brain Injury

doi: 10.3390/antiox14060640

Figure Lengend Snippet: Exo70 overexpression reinforces NMDAR synaptic availability upon mTBI induction. Samples from the dorsal hippocampus were analyzed by Western blot. Here, 30 µg of protein samples were resolved in 10% SDS-PAGE and transferred to PVDF membranes. Tubulin and Actin were used as loading controls. ( A ) Characterization of the lentiviral expression in all experimental groups using GFP and HA antibodies. ( B ) Densitometric analysis of HA-Exo70. ( C ) Western blot analyzing NMDAR synaptic localization after mTBI in injected mice. Samples were analyzed using GluN2B p1472, GluN2B p1336, and total GluN2B antibodies. ( D – F ) Densitometric analysis normalized with loading controls before normalization with Sham-GFP control samples. ( G ) Western blot analyzing NMDAR synaptic-signaling-related proteins after mTBI induction in Exo70-overexpressing samples. Samples were analyzed using pCREB and pERK1/2 antibodies. ( H , I ) The graphs show the densitometric analysis normalized with loading controls before normalization with Sham-GFP control samples. White circles indicate individual experiments. Values represent means ± SEM, n = 5 mice per experimental group. Statistical differences were calculated by two-way ANOVA, followed by post hoc Bonferroni’s test. * p < 0.05, ** p < 0.01, *** p < 0.001, ns: statistically non-significant difference.

Article Snippet: Primary antibodies: rabbit Exo70 1:1000 (Proteintech, Rosemont, IL, USA), goat GFP 1:1000 WB; 1:500 IHC (NovusBiologicals, Centennial, CO, USA), mouse HA 1:1000 (Cell Signalling, Danvers, MA, USA), mouse tubulin 1:1000 (NovusBio, Centennial, CO, USA), mouse β-actin 1:1000 (Sigma, Camas, WA, USA), rabbit GluN2B 1:1000 (Invitrogen, Waltham, MA, USA), rabbit GluN2B pY1472 1:1000 (Cell Signalling, Danvers, MA, USA), rabbit GluN2B pY1336 1:1000 (Invitrogen, Waltham, MA, USA), rabbit pCREB 1:1000 (NovusBiologicals, Centennial, CO, USA), rabbit pERK1/2 1:1000 (ThermoFisher Scientific, Waltham, MA, USA), mouse ERK1/2 1:1000 (ThermoFisher Scientific, Waltham, MA, USA).

Techniques: Over Expression, Western Blot, SDS Page, Expressing, Injection, Control

Journal: Cell reports

Article Title: CXCR4 Signaling Has a CXCL12-Independent Essential Role in Murine MLL-AF9 -Driven Acute Myeloid Leukemia

doi: 10.1016/j.celrep.2020.107684

Figure Lengend Snippet:

Article Snippet: Anti mouse ERK1/2 – BV421 (pT202/pY204) (Clone 20A) , BD Biosciences , Cat# 561991; RRID: AB_10895978.

Techniques: Virus, Recombinant, Cell Culture, Plasmid Preparation, ROS Assay, RNA Sequencing Assay, Software